A Guide to the DBCO-Reagents – Know about DBCO Acid and Amino

The field of biochemistry is vast and use of several reagents has been noted in this field of science. These reagents are not only expensive, but highly adhesive too, if they go in wrong hands or they are used by wrong persons. They can cause several damages to our different body parts, if they are inhaled or tasted or swallowed. Thus, while working with the reagents, maintaining utter safety has been required, and it should be the top priority of the students of biochemistry. These reagents have some positive sides too. They have been used successfully to attain some of the major findings of biochemistry. Several medicines or different causing behind physical reactions and many more things have been derived from the reactions of these reagents.

A Guide to DBCO Reagents

DBCO represent the dibenzocyclooctyne group of reactors that allow click chemistry copper free reactions with live cells. These reagents can also get in reactions with the whole organisms and even with several non-living samples. DBCO groups are considered as label molecules that are used vastly in the major biochemistry experiments. When it comes to DBCO reagents, several types of them can be found. The most common type is DBCO-azide, which contains a azide group (N3), which can react with alkynes. However, it has to be remembered that DBCO groups do not get into chemical reactions with hydroxyls or amino groups. Hydroxyls or amino groups have been found to be present naturally in various bio-molecules. DBCO group can mingle or get into chemical reactions with azide groups and at the end of the reaction in different occasions DBCO azide has been formed.

A Guide to DBCO-Acid and Amine

DBCO-acid has been characterized or recognized by its biocompatibility. The click reaction for this substance can occur under mild conditions and for the reaction, no catalysts have been required. DBCO acid is highly chemoselective and it does not react with the various functional groups that can be found in biological samples. It does not react, but it can conjugate. All DBCO groups have been noted to have more or less same characteristics to offer. Properties of DBCO-amine do not deviate much with the basic properties of acid group.

What Is DBCO-Cy5?

For chemo-selective labeling and for the recognition of the azido tagged molecules, DBCO activated fluorescent dyes have been often observed to be used in the modern bio-chemistry reactions. DBCO-Cy5 has been recognized through its high soluble power in water miscible substances.


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DBCO group can mingle or get into chemical reactions with azide groups and at the end of the reaction in different occasions  DBCO-Amine has been formed. To get more details click here.